Reptile and Amphibian Disinfection Protocol
Disease transmission between reptile and amphibian populations is becoming an important issue. To prevent disease transmission between study/collection locations that are not "water connected" or that animals don't move freely between, the following disinfection protocols are provided. The procedure should be completed on all gear/equipment that may have touched site water or was used to handle animals, including but not limited to:
- Dip nets
- Rulers and other instruments
- Specimen bags/containers
Materials that will be needed for disinfecting equipment include:
- Plastic bucket
- Gallon of chlorine bleach
- Two stiff scrub brushes, one for sterilization and one for cleaning off mud/dirt
- Spray bottle
- Before leaving the site, wash off as much of the mud/dirt on equipment and gear in the site's water source removing any vegetation or detritus attached.
- Fill bucket with two gallons (eight quarts/32 cups) clear water.
- Add 36 capfuls (1 cup) of bleach (3% concentration) and stir to mix.
- Rinse off any equipment used in the bleach solution. NOTE: Do all sterilizing with bleach solution well away from streams or ponds
- Dip shoes in solution and scrub and shake off.
- Dip and scrub waders in bucket or lay them on the ground and pour solution on them. A spray bottle can also be used to apply solution.
- Sterilize brushes in solution.
- If possible, save any remaining sterilization solution in a sealable container for future use. If solution must be discarded, dispose of on asphalt, cement or hard roadbed, away from any water bodies.
- If at all possible, allow gear and equipment to dry completely before use at next site.
NOTE: While it is still recommended, permittees collecting or working in a single site location are not obligated to disinfect gear/equipment. This includes nature center permittees working solely on their own property.