Disinfection Protocol

Reptile and Amphibian Disinfection Protocol
Disease transmission between reptile and amphibian populations is becoming an important issue. To prevent disease transmission between study/collection locations that are not "water connected" or that animals don't move freely between, the following disinfection protocols are provided. The procedure should be completed on all gear/equipment that may have touched site water or was used to handle animals, including but not limited to:

  • Waders
  • Shoes/boots
  • Dip nets
  • Rulers and other instruments
  • Specimen bags/containers
  • Traps

Materials that will be needed for disinfecting equipment include:

  • Plastic bucket
  • Gallon of chlorine bleach
  • Two stiff scrub brushes, one for sterilization and one for cleaning off mud/dirt
  • Spray bottle


  1. Before leaving the site, wash off as much of the mud/dirt on equipment and gear in the site's water source removing any vegetation or detritus attached.
  2. Fill bucket with two gallons (eight quarts/32 cups) clear water.
  3. Add 36 capfuls (1 cup) of bleach (3% concentration) and stir to mix.
  4. Rinse off any equipment used in the bleach solution. NOTE: Do all sterilizing with bleach solution well away from streams or ponds
    1. Dip shoes in solution and scrub and shake off.
    2. Dip and scrub waders in bucket or lay them on the ground and pour solution on them. A spray bottle can also be used to apply solution.
    3. Sterilize brushes in solution.
  5. If possible, save any remaining sterilization solution in a sealable container for future use. If solution must be discarded, dispose of on asphalt, cement or hard roadbed, away from any water bodies.
  6. If at all possible, allow gear and equipment to dry completely before use at next site.
  7. NOTE: While it is still recommended, permittees collecting or working in a single site location are not obligated to disinfect gear/equipment. This includes nature center permittees working solely on their own property.